PTPN21 Antibody

Product Discontinued

PTPN21 Antibody Summary

• Immunogen: Synthetic peptide C-RVLIQFLKSSRLI, representing the C-Terminus of the human protein according to NP_008970.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPN21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: This antibody can be used for ELISA and Western Blot. In Western Blot, Preliminary experiments gave no signal but low background in Human Lung and Muscle lysates. Not tested in other applications. Recommended Starting Dilution:* ELISA (peptide): 1:4,000 WB: 1ug/ml *Optimal dilutions should be determined by the end user.

Reactivity Notes

Expected from sequence similarity: Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for PTPN21 Antibody

• dJ1175B15.2 (protein tyrosine phosphatase, non-receptor type 21)
• EC 3.1.3
• EC 3.1.3.48
• protein tyrosine phosphatase, non-receptor type 21
• Protein-tyrosine phosphatase D1
• PTPD1
• PTPD1protein tyrosine phosphatase D1
• PTPN21
• PTPRL10
• tyrosine-protein phosphatase non-receptor type 21

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal domain, similar to cytoskeletal- associated proteins including band 4.1, ezrin, merlin, and radixin. This PTP was shown to specially interact with BMX/ETK, a member of Tec tyrosine kinase family characterized by a multimodular structures including PH, SH3, and SH2 domains. The interaction of this PTP with BMX kinase was found to increase the activation of STAT3, but not STAT2 kinase. Studies of the similar gene in mice suggested the possible roles of this PTP in liver regeneration and spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN21
• Entrez:
  • 11099(Human)
• Uniprot:
  • NP_008970.(Human)