PTGER3 Overexpression Lysate (Native)


Western Blot: Prostaglandin E Receptor EP3 Overexpression Lysate (Adult Normal) [NBP2-04450] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Prostaglandin E more

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

PTGER3 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_942009. The protein contains a C-terminal DDK tag.
Homo sapiens Prostaglandin E Receptor EP3, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for PTGER3

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PTGER3 Overexpression Lysate (Native)

  • EP3
  • EP3EP3e
  • EP3-I
  • EP3-II
  • EP3-III
  • EP3-IV
  • MGC141828
  • MGC141829
  • MGC27302
  • PGE receptor EP3 subtype
  • PGE receptor, EP3 subtype
  • PGE2 receptor EP3 subtype
  • PGE2-R
  • prostaglandin E receotor EP3 subtype 3 isoform
  • prostaglandin E receptor 3 (subtype EP3)
  • prostaglandin E2 receptor EP3 subtype
  • prostaglandin receptor (PGE-2)
  • Prostanoid EP3 receptor
  • PTGER3


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PTGER3 Lysate (NBP2-04450). (Showing 1 - 1 of 1 FAQ).

  1. I received an e-mail about some complimentary antibody samples that are available. We work with Ptger3 and have tried several antibodies (Santa Cruz, Abcam, Cayman) but haven't found an antibody that works. I was wondering if you would be willing to send us a complimentary sample of your Ptger3 antibody to test? If it works, we will likely buy a decent amount.
    • A list of our Ptger3 antibodies can be found here. It is company policy that we do not give out free samples as we have a 100% guarantee on all of our products for the applications and species listed on the datasheet. The email you received about free samples only applies to the antibodies listed in the email, of which Ptger3 is not listed.

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Gene Symbol PTGER3