PSMB7 Antibody (787807)

Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, beta TC-6 mouse beta cell insulinoma cell line, AR42J human acinar pancreatic cancer cell line, HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, MCF-7 human breast cancer cell line, and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human PSMB7 Monoclonal Antibody (Catalog # MAB7590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PSMB7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 787807
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PSMB7 Antibody (787807) Summary

• Immunogen: E. coli-derived recombinant human PSMB7
  Thr44-Ser277
  Accession # Q99436
• Specificity: Detects human PSMB7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PSMB10 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMB7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PSMB7 Antibody (787807)

• Macropain Chain Z
• Proteasome Subunit Z
• PSMB7
• Z

Background

PSMB7 (proteasome subunit, beta-type 7), also called proteasome subunit Z, is a widely expressed, non-catalytic component of the 20S proteasome core within the 26S proteasome that stabilizes late stages of proteasome assembly. Human PSMB7 is synthesized as a 277 amino acid (aa) protein with a 43 aa propeptide and a 234 active protein that contains one potential tyrosine phosphorylation site. Active human PSMB7 shares 97% aa sequence identity with mouse and rat PSMB7. Total PSMB7 expression is positively correlated with chemotherapy resistance in human breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSMB7 Antibody (MAB7590)(2)

Publications using MAB7590

• J Du, S Babik, Y Li, KK Deol, SJ Eyles, J Fejzo, M Tonelli, E Strieter A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation Elife, 2022-04-22;11(0):. 2022-04-22 [PMID: 35451368] (Western Blot, Human)
• Kirandeep K. Deol, Sean O. Crowe, Jiale Du, Heather A. Bisbee, Robert G. Guenette, Eric R. Strieter Proteasome-Bound UCH37/UCHL5 Debranches Ubiquitin Chains to Promote Degradation Molecular Cell 2020-12-01 [PMID: 33156996]

Bioinformatics

• Gene Symbol: PSMB7
• Uniprot:
  • Q99436(Human)