Protocadherin-8 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

Protocadherin-8 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
  Lys30-Pro749
  Accession # O95206
• Specificity: Detects human Protocadherin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Protocadherin-10 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protocadherin-8 Antibody [Alexa Fluor® 350]

• Arcadlin
• PAPC
• PCDH8
• protocadherin 8
• Protocadherin8
• Protocadherin-8

Background

Protocadherin 8 (PCDH-8, Arcadlin) is a 110-115 kDa group F member of the protocadherin family of molecules. It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca⁺⁺-dependent homophilic complexes. In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP. Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length. It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region. There are six cadherin domains in the ECD (aa 30-723). Multiple splice variants exist. Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527. Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.