Protocadherin-12 Antibody - BSA Free

Western Blot: Protocadherin-12 Antibody [NBP1-77002] - Analysis of PCDH12 in K562 cell lysate with PCDH12 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry: Protocadherin-12 Antibody - BSA Free [NBP1-77002] - Immunohistochemistry of Protocadherin-12 in rat lung tissue with Protocadherin-12 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Protocadherin-12 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human PCDH12. The immunogen is located within amino acids 30 - 80 of PCDH12. Amino Acid Squence: KLSQELGREERRRQAGA
• Specificity: This antibody is predicted to not cross-react with PCDH18.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCDH12
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Protocadherin-12 Peptide (NBP1-77002PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Protocadherin-12 Antibody - BSA Free

• PCDH12
• protocadherin 12
• Protocadherin12
• Protocadherin-12
• Vascular cadherin-2
• vascular endothelial cadherin 2
• Vascular endothelial cadherin-2
• VECAD2
• VE-cad-2
• VE-cadherin 2
• VE-cadherin-2
• VE-cadherin-2protocadherin-12

Background

Protocadherins comprise the largest group within the cadherin family of calcium-dependent cell-cell adhesion molecules. Protocadherin 12 (PCDH12) was initially identified through PCR screening of mouse heart microvascular endothelial cell RNA; further experiments revealed its mRNA to be strongly expressed in highly vascularized organs such as lung and kidney, in addition to glycogen-rich trophoblasts in the placenta. PCDH12-null mice are viable and fertile, but show reduced placental and embryonic sizes when compared to wild-type mice. Further studies showed significant expression changes in 2,289 genes, including those involved in tissue morphogenesis, angiogenesis, cell-matrix adhesion and migration, immune response and chromatin remodeling. This antibody is predicted to not cross-react with PCDH18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protocadherin-12 Antibody (NBP1-77002)(1)

Publication using NBP1-77002

• Sobel RA, Eaton MJ, Jaju PD et al. Anti-Myelin Proteolipid Protein Peptide Monoclonal Antibodies Recognize Cell Surface Proteins on Developing Neurons and Inhibit Their Differentiation J. Neuropathol. Exp. Neurol. 2019-09-01 [PMID: 31400116] (IHC-P, Rat)

Bioinformatics

• Gene Symbol: PCDH12
• Uniprot:
  • NP_057664(Human)