Protein phosphatase 1F Antibody (6D11) - BSA Free

Western Blot: Protein phosphatase 1F Antibody (6D11) [NBP2-42648] - The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSR2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 6D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Protein phosphatase 1F Antibody (6D11) - BSA Free Summary

• Description: Novus Biologicals Mouse Protein phosphatase 1F Antibody (6D11) - BSA Free (NBP2-42648) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Protein phosphatase 1F (1-454aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1F
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Protein phosphatase 1F Antibody (6D11) - BSA Free

• Ca(2+)/calmodulin-dependent protein kinase phosphatase
• CaMKPaseCaM-kinase phosphatase
• EC 3.1.3.16
• FEM-2
• hFEM-2
• KIAA0015POPX2CAMKP
• Partner of PIX 2
• PP2C phosphatase
• Protein fem-2 homolog
• protein phosphatase 1F (PP2C domain containing)
• protein phosphatase 1F
• protein phosphatase, Mg2+/Mn2+ dependent, 1F

Background

Protein phosphatase 1F is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1F
• Uniprot:
  • NP_055449(Human)