Protein phosphatase 1F Antibody (1G10) - Azide and BSA Free

Western Blot: Protein phosphatase 1F Antibody (1G10) [H00009647-M02] - Analysis of PPM1F expression in transfected 293T cell line by PPM1F monoclonal antibody (M02), clone 1G10.Lane 1: PPM1F transfected lysate(49.8 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: Protein phosphatase 1F Antibody (1G10) [H00009647-M02] - Analysis of PPM1F transfected lysate using anti-PPM1F monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PPM1F MaxPab rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 1G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Protein phosphatase 1F Antibody (1G10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Protein phosphatase 1F Antibody (1G10) - Azide and BSA Free (H00009647-M02) is a monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PPM1F (NP_055449, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSGAPQKSSPMASGAEETPGFLDTLLQDFPALLNPEDPLPWKAPGTVLSQEEVEGELAELAMGFLGSRKAPPPLAAALAHEAVSQLLQTDLSEFRKLPR
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1F
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot
• Application Notes: This antibody is useful for ELISA, Western Blot

Reactivity Notes

This product is reactive against Human.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Protein phosphatase 1F Antibody (1G10) - Azide and BSA Free

• Ca(2+)/calmodulin-dependent protein kinase phosphatase
• CaMKPaseCaM-kinase phosphatase
• EC 3.1.3.16
• FEM-2
• hFEM-2
• KIAA0015POPX2CAMKP
• Partner of PIX 2
• PP2C phosphatase
• Protein fem-2 homolog
• protein phosphatase 1F (PP2C domain containing)
• protein phosphatase 1F
• protein phosphatase, Mg2+/Mn2+ dependent, 1F

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1F
• Uniprot:
  • NP_055449(Human)