Proteasome 26S S5 Antibody - BSA Free

Western Blot: Proteasome 26S S5 Antibody [NB120-3319] - Analysis of proteasome 19S subunit S5b on purified 26S proteasome.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Proteasome 26S S5 Antibody - BSA Free Summary

• Immunogen: Synthetic Peptide: L(485) S E G P Y Y V K P V S T T A V E G A E(504)
• Specificity: NB120-3319 immunizing peptide corresponds to amino acid residues 485-504 from human proteasome 19S subunit S5b protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMD5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1.0 ug/ml
• Application Notes: Useful in WB. WB: Detects an approx. 55 kDa protein representing purified human proteasome 19S subunit S5b.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Proteasome 26S S5 Antibody - BSA Free

• KIAA007226S proteasome non-ATPase regulatory subunit 5
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 5,26S proteasome subunit S5B
• S5BMGC23145,26S protease subunit S5 basic

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMD5