Proteasome 19S S7 Antibody

Western Blot: Proteasome 19S S7 Antibody [NB120-3322] - Analysis of proteasome 19S subunit S7 on HeLa cell lysate.

Product Discontinued

Proteasome 19S S7 Antibody Summary

• Immunogen: Human recombinant proteasome 19S subunit S7.
• Specificity: Detects proteasome 19S subunit S7.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMC2
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 47 kDa protein representing proteasome 19S subunit S7 from HeLa cell lysate.
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for Proteasome 19S S7 Antibody

• 26S protease regulatory subunit 7
• 26S proteasome AAA-ATPase subunit RPT1
• MGC3004
• MSS1ATPase, 2
• proteasome (prosome, macropain) 26S subunit, ATPase, 2
• Protein MSS1
• putative protein product of Nbla10058

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMC2
• Entrez:
  • 5701(Human)
• Uniprot:
  • P35998(Human)