Prostasin/Prss8 Antibody (530622) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: 530622
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Prostasin/Prss8 Antibody (530622) [Alexa Fluor® 350] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Prostasin/Prss8
  Ala33-Gly319
  Accession # Q16651
• Specificity: Detects human Prostasin/Prss8 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse Prostasin/Prss8 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prostasin/Prss8 Antibody (530622) [Alexa Fluor® 350]

• CAP1
• channel-activating protease 1
• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.120
• Prostasin
• protease, serine, 8
• Prss8
• Serine protease 8

Background

Prostasin, also known as Prss8 and channel activating protease 1, is a 40 kDa serine protease with trypsin-like substrate specificity that is a member of the peptidase S1 family. The enzyme is synthesized from a 343 amino acid (aa) preproenzyme that contains a 29 aa signal peptide, a propeptide (aa 30-32) and a 290 aa mature chain. A second propeptide (aa 323-343) contains a 21 aa transmembrane region (GPI anchor) which can be proteolytically processed to generate a secreted form of the enzyme. The mature chain forms a heterodimer made up of a light chain (aa 33-44) and a heavy chain (aa 45-322) that are connected by disulfide bonds. In addition, aa 45-286 constitute a peptidase S1 domain, and aa 159 is a potential site for N-linked glycosylation. Mature human Prostasin is 79% and 78% aa identical to mature mouse and rat Prostasin, respectively. Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, salivary gland, and pancreas. It activates amiloride-sensitive sodium channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.