PRKD3/nPKC nu Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

PRKD3/nPKC nu Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PRKD3/nPKC nu
  Ile465-Val559
  Accession # O94806
• Specificity: Detects human PRKD3/nPKC nu in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRKD3/nPKC nu Antibody [Alexa Fluor® 488]

• EC 2.7.11
• EC 2.7.11.13
• EPK2
• EPK2PKD3
• nPKC nu
• nPKC-nu
• PKC nu
• PKC-NU
• PRKCN
• PRKCNnPKC-NU
• PRKD3
• Protein kinase C nu type
• protein kinase C, nu
• protein kinase D3
• Protein kinase EPK2
• protein-serine/threonine kinase
• serine/threonine-protein kinase D3

Background

PKC nu (Protein kinase C‑nu; also PKD3 and PRKD3) is a 105‑110 kDa member of the PKD subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in highly diverse cell types such as pancreatic aciner cells, skeletal muscle cells, B cells and sensory neurons, and it is activated by PKC‑mediated phosphorylation. Active PKC nu associates with membrane compartments, and appears to participate with VAMP2 in cargo transport and vesicular trafficking. It also acts on class II HDACs in the nucleus, resulting in gene transcription. Human PKC nu is 890 amino acids (aa) in length. It contains two DAG‑type zinc finger regions (aa 154‑204 and 271‑321), one PH domain (aa 416‑532) and a large protein kinase domain (aa 576‑832). There are at least 25 utilized Ser/Thr phosphorylation sites that span the length of the molecule. PKC nu possesses one potential splice variant that shows a 17 aa substitution for aa 595‑890. Over aa 465‑559, human PKC nu is 92% aa identical to mouse PKC nu .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O94806(Human)