PRKD3/nPKC nu Antibody [Alexa Fluor® 594]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

PRKD3/nPKC nu Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human PRKD3/nPKCν
  Ile465-Val559
  Accession # O94806
• Specificity: Detects human PRKD3/nPKC nu in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PKC nu (Protein kinase C‑nu; also PKD3 and PRKD3) is a 105‑110 kDa member of the PKD subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in highly diverse cell types such as pancreatic aciner cells, skeletal muscle cells, B cells and sensory neurons, and it is activated by PKC‑mediated phosphorylation. Active PKC nu associates with membrane compartments, and appears to participate with VAMP2 in cargo transport and vesicular trafficking. It also acts on class II HDACs in the nucleus, resulting in gene transcription. Human PKC nu is 890 amino acids (aa) in length. It contains two DAG‑type zinc finger regions (aa 154‑204 and 271‑321), one PH domain (aa 416‑532) and a large protein kinase domain (aa 576‑832). There are at least 25 utilized Ser/Thr phosphorylation sites that span the length of the molecule. PKC nu possesses one potential splice variant that shows a 17 aa substitution for aa 595‑890. Over aa 465‑559, human PKC nu is 92% aa identical to mouse PKC nu .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.