PRKCSH Antibody (4Z3K3)

Western Blot: PRKCSH Antibody (4Z3K3) [NBP3-15318] - Western blot analysis of extracts of various cell lines, using PRKCSH antibody (NBP3-15318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

• Reactivity: Hu
• Applications: WB
• Clone: 4Z3K3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRKCSH Antibody (4Z3K3) Summary

• Description: Novus Biologicals Rabbit PRKCSH Antibody (4Z3K3) (NBP3-15318) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 429-528 of human PRKCSH (P14314). PFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PRKCSH
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PRKCSH Antibody (4Z3K3)

• AGE-R2,80K-H protein
• G19P1AGE-binding receptor 2
• glucosidase 2 subunit beta
• Glucosidase II subunit beta
• glucosidase II, beta subunit
• hepatocystin
• PCLD
• PKCSH
• PLD1
• Protein kinase C substrate 60.1 kDa protein heavy chain
• protein kinase C substrate 80K-H
• protein kinase C substrate, 80 Kda protein

Background

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKCSH