PRAK Antibody (3D7) - Azide and BSA Free

Western Blot: PRAK Antibody (3D7) [H00008550-M02] - Analysis of MAPKAPK5 expression in transfected 293T cell line by MAPKAPK5 monoclonal antibody (M02), clone 3D7.Lane 1: MAPKAPK5 transfected lysate(54 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: PRAK Antibody (3D7) [H00008550-M02] - Analysis of monoclonal antibody to MAPKAPK5 on HeLa cell . Antibody concentration 10 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PRAK Antibody (3D7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAPKAPK5 (AAH47284, 371 a.a. ~ 471 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKVDRLKLAEIVKQVIEEQTTSHESQ
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPKAPK5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: It has been used for ELISA and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PRAK Antibody (3D7) - Azide and BSA Free

• EC 2.7.11
• MAP kinase-activated protein kinase 5
• MAPK-activated protein kinase 5
• MAPKAP kinase 5
• MAPKAPK-5
• mitogen-activated protein kinase-activated protein kinase 5
• p38-regulated/activated protein kinase
• PRAKEC 2.7.11.1

Background

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAPK5
• Uniprot:
  • AAH47284(Human)