PPP2R5A Antibody

Product Discontinued

PPP2R5A Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human protein phosphatase 2, regulatory subunit B (B56), alpha isoform using the numbering given in entry NP_006234.1 (GeneID 5525).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP2R5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP2R5A Antibody

• B56A
• MGC131915
• PP2A B subunit isoform B56-alpha
• PP2A B subunit isoform B'-alpha
• PP2A B subunit isoform PR61-alpha
• PP2A B subunit isoform R5-alpha
• PP2A, B subunit, B' alpha isoform
• PP2A, B subunit, B56 alpha isoform
• PP2A, B subunit, PR61 alpha isoform
• PP2A, B subunit, R5 alpha isoform
• PR61A
• PR61alpha
• protein phosphatase 2, regulatory subunit B (B56), alpha isoform
• protein phosphatase 2, regulatory subunit B', alpha isoform
• protein phosphatase 2, regulatory subunit B', alpha
• serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alphaisoform
• serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform

Background

PPP2R5A belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity and might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R5A Antibody (NB100-41412)(3)

Publications using NB100-41412

• Varadkar P, Abbasi F, Takeda K et al. PP2A-B56g is required for an efficient spindle assembly checkpoint. Cell Cycle. 2017-06-18 [PMID: 28562161]
• Chen L, Lai Y, Zhu X et al. The role of specific PP2A complexes in the dephosphorylation of gamma-H2AX. J Cell. Sci. 2014-09-18 [PMID: 25236601]
• Ruediger R, Ruiz J, Walter G et al. Human cancer-associated mutations in the Aalpha subunit of protein phosphatase 2A increase lung cancer incidence in Aalpha knock-in and knockout mice. Mol Cell Biol 2011-09-01 [PMID: 21791616]

Bioinformatics

• Gene Symbol: PPP2R5A
• Entrez:
  • 5525(Human)
  • 226849(Mouse)
• Uniprot:
  • Q15172(Human)