PPP2R3A Antibody

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NB100-93429] - Staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PPP2R3A Antibody Summary

• Description: Novus Biologicals Goat PPP2R3A Antibody (NB100-93429) is a polyclonal antibody validated for use in IHC and ELISA. Anti-PPP2R3A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QKDVENDGPEPSD corresponding to internal region according to NP_002709.2, NP_871626.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_002709.2; NP_871626.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP2R3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave an approx. 80 kDa band in human skeletal muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130 kDa band according to NP_002709.2 and of 61.1 kDa band according to NP_871626.1. The 80 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP2R3A Antibody

• PP2A subunit B isoform R3 isoform
• PP2A subunit B isoforms B72/B130
• PP2A subunit B isoforms B'-PR72/PR130
• protein phosphatase 2 (formerly 2A), regulatory subunit B' (PR 72), alphaisoform and (PR 130), beta isoform
• protein phosphatase 2 (formerly 2A), regulatory subunit B', alpha
• protein phosphatase 2, regulatory subunit B', alpha
• serine/threonine-protein phosphatase 2A regulatory subunit B' subunit alpha
• subunit B, R3 isoform

Background

Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R3A Antibody (NB100-93429)(1)

Publication using NB100-93429

• Zhang S, Cagatay T, Amanai M et al. Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol 2007-06-01 [PMID: 17438140]

Bioinformatics

• Gene Symbol: PPP2R3A
• Entrez:
  • 5523(Human)
• Uniprot:
  • Q06190(Human)