PPP2R1A Antibody

Western Blot: PPP2R1A Antibody [NB100-1497] - Staining of Human Thymus (A) and Human Lymph node (B) lysates (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PPP2R1A Antibody Summary

• Immunogen: Peptide with sequence C-KYFAQEALTVLSLA corresponding to C-Terminus according to NP_055040.2.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP2R1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:14000
  • Western Blot 2 - 6 ug/mL
• Control:

  PP2A alpha Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPP2R1A Antibody

• Medium tumor antigen-associated 61 kDa protein
• MGC786
• PP2A subunit A isoform PR65-alpha
• PP2A subunit A isoform R1-alpha
• PP2AAALPHA
• PP2A-Aalpha
• PR65A
• protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alphaisoform
• protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
• protein phosphatase 2, regulatory subunit A, alpha
• serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alphaisoform
• serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alphaisoform

Background

Protein phosphatase 2A (PP2A) is the most abundant phosphatase specific for serine and threonine phosphorylation in mammalian cells. Dephosphorylation of substrates targeted by PP2A plays a vital role in a large variety of cellular processes including signal transduction, regulation of the cell cycle, gene expression and development. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit alpha serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory beta subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPP2R1A Antibody (NB100-1497)(2)

Publications using NB100-1497

• Tang Y, Fang G, Guo F et al. Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer Cancer Cell 2020-06-10 [PMID: 32589942]
• Hong Y, Sarge KD. Regulation of protein phosphatase 2A activity by heat shock transcription factor 2. J Biol Chem 1999-05-07 [PMID: 10224043]

Bioinformatics

• Gene Symbol: PPP2R1A
• Entrez:
  • 5515(Human)
• Uniprot:
  • NP_055040.(Human)