PPIL3 Overexpression Lysate

Images

 
Western Blot: PPIL3 Overexpression Lysate (Adult Normal) [NBL1-14653] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPIL3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PPIL3 Overexpression Lysate Summary

Description

PPIL3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC217389

Accession#: NM_131916

Protein Tag: C-MYC/DDK
Gene
PPIL3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PPIL3 Overexpression Lysate

  • Cyclophilin J
  • cyclophilin-like protein 3
  • Cyclophilin-like protein PPIL3
  • CyPJ
  • EC 5.2.1.8
  • peptidyl-prolyl cis-trans isomerase-like 3
  • peptidylprolyl cis-trans isomerase-like protein 3
  • peptidylprolyl isomerase (cyclophilin)-like 3
  • PPIase
  • PPIase-like protein 3
  • Rotamase PPIL3

Background

This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (PPIL3c) contains alternate segments in the coding region and the 5' UTR, compared to variant PPIL3a, resulting in a shorter isoform (PPIL3b). Variants PPIL3b and PPIL3c encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PPIL3