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Recombinant Human PPIG Protein

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SDS-Page: Recombinant Human PPIG Protein [NBP1-50830] - 15 % SDS-PAGE (3ug)
SDS-Page: Recombinant Human PPIG Protein [NBP1-50830] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

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Recombinant Human PPIG Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-175 of Human PPIG

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV VREIENQKTD AASKPFAEVR ILSCG
Details of Functionality
Specific activity is > 600 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PPIG
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human PPIG Protein

  • CARS-cyclophilin
  • CARS-CypPPIase G
  • CASP10
  • Clk-associating RS-cyclophilin
  • Cyclophilin G
  • CYP
  • EC 5.2.1.8
  • MGC133241
  • peptidyl-prolyl cis-trans isomerase G
  • peptidylprolyl isomerase G (cyclophilin G)
  • peptidyl-prolyl isomerase G (cyclophilin G)
  • Peptidyl-prolyl isomerase G
  • Rotamase G
  • SCAF10
  • SR-cyclophilin
  • SRCyp
  • SR-cyp
  • SR-related CTD-associated factor 10

Background

: Peptidyl-prolyl cis-trans isomerase G (PPIG), also known as Cyclophilin G, is a member of peptidyl-prolyl cis-trans isomerase family (PPIases). This protein catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is localized to the nuclear speckles, a nuclear compartment rich in splicing factors, and interacts with the splicing factors SC35 and pinin. Cyclophilin G also may play an important role in the regulation of pre-mRNA splicing. Recombinant human Cyclophilin G, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PPIG