PP5 Antibody (12F7) - BSA Free

Western Blot: PP5 Antibody (12F7) [NBP2-59308] - Analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Lane 1: MW Ladder. Lane 2: Human A431 (15 ug). Lane 3: Human HEK293 (15 ug). Lane 4: Human Jurkat (15 ug). Load: 15 ug protein. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.

Immunocytochemistry/Immunofluorescence: PP5 Antibody (12F7) [NBP2-59308] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.

Immunocytochemistry/Immunofluorescence: PP5 Antibody (12F7) [NBP2-59308] - Tissue: HEK293. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 12F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

PP5 Antibody (12F7) - BSA Free Summary

• Immunogen: Full length human PP5 protein
• Localization: Nucleus , Cytoplasm
• Specificity: Detects 58kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP5C
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for PP5 Antibody (12F7) - BSA Free

• EC 3.1.3.16
• PP5
• PP5FLJ36922
• PPP5
• PPP5C
• PPT
• PP-T
• protein phosphatase 5, catalytic subunit
• Protein phosphatase T
• Serine/Threonine phosphatase
• serine/threonine-protein phosphatase 5

Background

It may play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, it dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP5C