Recombinant Human PP4/PPP4C His Protein

SDS-Page: Recombinant Human PPP4C Protein [NBP2-23383]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human PP4/PPP4C His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-307 of Human PPP4C
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEISDL DRQIEQLRRC ELIKESEVKA LCAKAREILV EESNVQRVDS PVTVCGDIHG QFYDLKELFR VGGDVPETNY LFMGDFVDRG FYSVETFLLL LALKVRYPDR ITLIRGNHES RQITQVYGFY DECLRKYGSV TVWRYCTEIF DYLSLSAIID GKIFCVHGGL SPSIQTLDQI RTIDRKQEVP HDGPMCDLLW SDPEDTTGWG VSPRGAGYLF GSDVVAQFNA ANDIDMICRA HQLVMEGYKW HFNETVLTVW SAPNYCYRCG NVAAILELDE HLQKDFIIFE AAPQETRGIP SKKPVADYFL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PPP4C
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 37.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PP4/PPP4C His Protein

• EC 3.1.3.16
• PP4
• PP4C
• PP4protein phosphatase X, catalytic subunit
• PPH3
• PPP4C
• PPX
• PP-X
• PPXPPH3
• protein phosphatase 4 (formerly X), catalytic subunit
• protein phosphatase 4, catalytic subunit
• Protein phosphatase X

Background

Serine/threonine-protein phosphatase 4 catalytic subunit, also known as PPP4C, belongs to the Serine/threonine-protein phosphatase catalytic subunits. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPP4C is a putative member of a novel PP family. The PPP4C may play a role in dephosphorylation and regulation of HDAC3. Recombinant human PPP4C protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PPP4C