PP2C gamma/PPM1G Antibody (OTI1C12)

Western Blot: PPM1G Antibody (1C12) [NBP2-03463] Analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1G monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: PPM1G Antibody (1C12) [NBP2-03463] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PPM1G.

Western Blot: PPM1G Antibody (1C12) [NBP2-03463] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF
• Clone: OTI1C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PP2C gamma/PPM1G Antibody (OTI1C12) Summary

• Immunogen: Full length human recombinant protein of human PPM1G (NP_817092) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 59.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PP2C gamma/PPM1G Antibody (OTI1C12)

• EC 3.1.3.16
• MGC1675
• MGC2870
• PP2C gamma
• PP2C, gamma
• PP2CG
• PP2CGAMMA
• PP2C-gamma
• PPM1C
• PPM1G
• PPP2CG
• Protein phosphatase 1C
• protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• protein phosphatase 1G
• protein phosphatase 2, catalytic subunit, gamma isoform
• protein phosphatase 2C gamma isoform
• Protein phosphatase 2C isoform gamma
• Protein phosphatase magnesium-dependent 1 gamma
• protein phosphatase, Mg2+/Mn2+ dependent, 1G

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1G
• Entrez:
  • 5496(Human)