PP2C gamma/PPM1G Antibody (528322) [Unconjugated]

Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human PP2C gamma/PPM1G Monoclonal Antibody (Catalog # MAB5595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C gamma/PPM1G at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 528322
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PP2C gamma/PPM1G Antibody (528322) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PP2C gamma
  Met1-Asp546
  Accession # O15355
• Specificity: Detects human PP2C gamma .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1G
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP2C gamma/PPM1G Antibody (528322) [Unconjugated]

• EC 3.1.3.16
• MGC1675
• MGC2870
• PP2C gamma
• PP2C, gamma
• PP2CG
• PP2CGAMMA
• PP2C-gamma
• PPM1C
• PPM1G
• PPP2CG
• Protein phosphatase 1C
• protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• protein phosphatase 1G
• protein phosphatase 2, catalytic subunit, gamma isoform
• protein phosphatase 2C gamma isoform
• Protein phosphatase 2C isoform gamma
• Protein phosphatase magnesium-dependent 1 gamma
• protein phosphatase, Mg2+/Mn2+ dependent, 1G

Background

Protein phosphatase 2C isoform gamma (PP2C gamma; also PPM1G) is a widely expressed, 75 kDa member of the PP2C family of Ser/Thr protein phosphatases. Human PP2C gamma is 546 amino acids (aa) in length. The apparent mass is much larger than the predicted mass of 59 kDa because of the presence of an acidic region (aa 259‑326) rich in glutamate and aspartate residues, causing the protein to move anomalously on SDS-PAGE. The protein contains numerous sites for phosphorylation by casein kinase II, particularly within the acidic domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1G
• Uniprot:
  • O15355(Human)