Novus Biologicals products are now on bio-techne.com

PP2C alpha/PPM1A Overexpression Lysate

Images

 
Western Blot: PPM1A Overexpression Lysate (Adult Normal) [NBL1-14656] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPM1A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PP2C alpha/PPM1A Overexpression Lysate Summary

Description

PP2C alpha/PPM1A Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202070

Accession#: NM_021003

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
PPM1A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
42.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PP2C alpha/PPM1A Overexpression Lysate

  • EC 3.1.3.16
  • FLJ42306
  • MGC9201
  • MMPa-2
  • MPPa-1
  • phosphatase 2C alpha
  • PP2C alpha
  • Pp2c1
  • PP2CA
  • PP2Calpha
  • PP2C-ALPHA
  • PPM1A
  • PPPM1A
  • protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
  • protein phosphatase 1A
  • protein phosphatase 2C alpha isoform
  • Protein phosphatase 2C isoform alpha
  • Protein phosphatase IA
  • protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (1) is the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB41051
Species: Hu, Mu
Applications: IHC, WB
AF4396
Species: Hu, Mu, Rt
Applications: WB
NBP1-31348
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF8691
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
AF3797
Species: Hu, Mu
Applications: ChIP, ICC, IHC, Simple Western, WB
NBP1-77836
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, Single-Cell Western, WB
AF1230
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
NBP2-37568
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-00764
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP1-81575
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-68874
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
H00010598-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-88207
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB17761
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP1-47839
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-87232
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for PP2C alpha/PPM1A Lysate (NBL1-14656) (0)

There are no publications for PP2C alpha/PPM1A Lysate (NBL1-14656).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PP2C alpha/PPM1A Lysate (NBL1-14656) (0)

There are no reviews for PP2C alpha/PPM1A Lysate (NBL1-14656). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PP2C alpha/PPM1A Lysate (NBL1-14656) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PP2C alpha/PPM1A Products

Research Areas for PP2C alpha/PPM1A Lysate (NBL1-14656)

Find related products by research area.

Blogs on PP2C alpha/PPM1A

There are no specific blogs for PP2C alpha/PPM1A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PP2C alpha/PPM1A Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol PPM1A