PP2A alpha Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 594

PP2A alpha Antibody [Alexa Fluor® 594] Summary

• Immunogen: Phosphopeptide containing human PP2A Y307 site
• Specificity: Detects human, mouse, and rat PP2A when phosphorylated at Y307. The antigen is identical to the PP4 Y305 phosphorylation site.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP2A alpha Antibody [Alexa Fluor® 594]

• EC 3.1.3.16
• PP2A
• PP2A-alpha
• PP2Ac
• PP2CA
• PP2Calpha
• PPP2CA
• protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• protein phosphatase 2, catalytic subunit, alpha isozyme
• protein phosphatase 2A catalytic subunit, alpha isoform
• Replication protein C
• RP-C
• serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
• serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Background

Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the C-terminus at Y307 of the catalytic subunit decreases the phosphatase activity of PP2A and reduces its ability to bind to proteins such as CD28 and the glutamate receptor GluR5. Phosphorylation of PP2A at Y307 is highest during M-phase and lowest during S-phase of the cell cycle. Transient PP2A phosphorylation at this site is also observed in growth factor-stimulated cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.