PP2A alpha Antibody (P6C7)

Images

 
Western Blot: PP2A alpha Antibody (P6C7) [NBP1-50338] - Analysis of anti-PPP2CA / PP2Ac antibody with mouse liver cytosol extract. Arrow indicates PP2CA(~43kDa).
Immunohistochemistry-Paraffin: PP2A alpha Antibody (P6C7) [NBP1-50338] - Analysis of anti-PPP2CA / PP2Ac antibody with human kidney at concentration 15 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC-P, IP
Clone
P6C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PP2A alpha Antibody (P6C7) Summary

Immunogen
Recombinant human PP2Ckappa protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PPP2CA
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:1000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry-Paraffin 15 ug/ml
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Purity
Protein G purified

Alternate Names for PP2A alpha Antibody (P6C7)

  • EC 3.1.3.16
  • PP2A
  • PP2A-alpha
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • PPP2CA
  • protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
  • protein phosphatase 2, catalytic subunit, alpha isozyme
  • protein phosphatase 2A catalytic subunit, alpha isoform
  • Replication protein C
  • RP-C
  • serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
  • serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Background

Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways (1-3). PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PPP2CA
Entrez
Uniprot