PP1 alpha/PPP1A Antibody (6D1)

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Western Blot: PP1 alpha/PPP1A Antibody (6D1) [NBP1-51600] - WB analysis of PPP1A in human cell lysate. Verified customer review from 1DegreeBio.
Western Blot: PP1 alpha/PPP1A Antibody (6D1) [NBP1-51600] - Western blot analysis using PPP1A mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
6D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PP1 alpha/PPP1A Antibody (6D1) Summary

Immunogen
Purified recombinant fragment of human PPP1A expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPP1CA
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-51600 in the following applications:

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for PP1 alpha/PPP1A Antibody (6D1)

  • EC 3.1.3.16
  • PP1 alpha
  • PP1alpha
  • PP-1AMGC15877
  • PPP1A
  • PPP1AMGC1674
  • PPP1CA
  • protein phosphatase 1, catalytic subunit, alpha isoform
  • protein phosphatase 1, catalytic subunit, alpha isozyme
  • serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
  • serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Background

Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine (Ser/Thr) protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favouring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PP1 alpha/PPP1A Antibody (NBP1-51600) (0)

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Review for PP1 alpha/PPP1A Antibody (NBP1-51600) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-51600:
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Western Blot PP1 alpha/PPP1A NBP1-51600
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reviewed by:
Andrew Cosgrove
WB Human 11/06/2013
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Summary

ApplicationWestern Blot
Sample TestedHuman Whole Cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlock with TBST + 5% milk for 1 hr

Primary Anitbody

Dilution Ratio1:1000 in TBST + 2% Milk for 1hr

Secondary Antibody

Secondary ConcentrationTBST + 2% milk for 30 mins

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PP1 alpha/PPP1A Antibody (NBP1-51600) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PP1 alpha/PPP1A Antibody (NBP1-51600)

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Diseases for PP1 alpha/PPP1A Antibody (NBP1-51600)

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Recent Reviews

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4
1
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2
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1
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Andrew Cosgrove
11/06/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol PPP1CA
Entrez