Recombinant Human POU6F1 His Protein

SDS-Page: POU6F1 Protein [NBP2-23357]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human POU6F1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-301 of Human POU6F1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPGISSQ ILTNAQGQVI GTLPWVVNSA SVAAPAPAQS LQVQAVTPQL LLNAQGQVIA TLASSPLPPP VAVRKPSTPE SPAKSEVQPI QPTPTVPQPA VVIASPAPAA KPSASAPIPI TCSETPTVSQ LVSKPHTPSL DEDGINLEEI REFAKNFKIR RLSLGLTQTQ VGQALTATEG PAYSQSAICR FEKLDITPKS AQKLKPVLEK WLNEAELRNQ EGQQNLMEFV GGEPSKKRKR RTSFTPQAIE ALNAYFEKNP LPTGQEITEI AKELNYDREV VRVWFCNRRQ TLKNTSKLNV FQIP

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: POU6F1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human POU6F1 His Protein

• Brain-specific homeobox/POU domain protein 5
• Brn-5
• BRN5brain-5
• mPOU homeobox protein
• MPOUbrn-5
• POU class 6 homeobox 1
• POU domain, class 6, transcription factor 1
• TCFB1Brain-5

Background

POU6F1 belongs to the POU transcription factor family. This protein is transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). In the embryo, it is expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Recombinant human POU6F1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: POU6F1