POU2F3 Antibody

Western Blot: POU2F3 Antibody [NBP1-49870] - Western blot analysis of POU2F3 in HEK293 lysate (35ug protein in RIPA buffer) using NBP1-49870 at 2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

POU2F3 Antibody Summary

• Immunogen: Peptide with sequence C-EPHLEASQHLPVPKH corresponding to internal region according to NP_055167.2.
• Epitope: EPHLEASQHLPVPKH - internal region
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POU2F3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in lysates of HEK293 and HepG2 (calculated MW of 47.4 kDa band according to NP_055167.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for POU2F3 Antibody

• Epoc-1
• MGC126698
• OCT11
• OCT-11
• OCT11FLJ40063
• Octamer-binding protein 11
• Octamer-binding transcription factor 11
• OTF11
• OTF-11
• PLA1
• PLA-1
• POU class 2 homeobox 3
• POU domain class 2, transcription factor 3
• POU domain, class 2, transcription factor 3
• POU transcription factor
• POU2F3
• Skn-1a
• Transcription factor PLA-1
• Transcription factor Skn-1

Background

POU2F3, also referred to as POU domain, class 2, transcription factor 3, is a transcription factor that belongs to the POU protein family. POU2F3 has a 436 amino acid long isoform, a 438 amino acid long isoform, and a 432 amino acid long isoform, all three of which are approximately 47kDa. POU2F3 is highly expressed in keratinocytes and the epidermis. Current research surrounding POU2F3 has shown possible interactions with diseases and disorders such as atherosclerosis, bipolar disorder, herpes, cervical cancer, breast cancer, and neuroendocrine tumors. POU2F3 has also been shown to interact with Bcl6, Ubiquitin and KAT3A/CBP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POU2F3 Antibody (NBP1-49870)(1)

Publication using NBP1-49870

• Kukimoto I, Mori S, Sato H et al. Transcription factor human Skn-1a enhances replication of human papillomavirus DNA through the direct binding to two sites near the viral replication origin. FASEB J 2008-06-01 [PMID: 18479461]

Bioinformatics

• Gene Symbol: POU2F3
• Entrez:
  • 25833(Human)
• Uniprot:
  • NP_055167.2(Human)