Porimin Antibody (297617) [Unconjugated]

MDA-MB-231 cells were stained with Mouse Anti-Human Porimin Monoclonal Antibody (Catalog # MAB3010, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 297617
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Porimin Antibody (297617) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Porimin
  His27-Ser149
  Accession # AAG21694
• Specificity: Detects human Porimin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMEM123
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Porimin Antibody (297617) [Unconjugated]

• KCT3
• KCT-3
• KCT3keratinocytes associated transmembrane protein 3
• Keratinocytes-associated transmembrane protein 3
• Porimin
• PORIMINporimin
• pro oncosis receptor inducing membrane injury
• Pro-oncosis receptor inducing membrane injury
• serine/threonine-rich receptor
• TMEM123
• transmembrane protein 123PORMIN

Background

Porimin is a widely expressed mucin-related transmembrane glycoprotein. Cross-linking of Porimin leads to membrane disruption and cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Porimin Antibody (MAB3010)(2)

Publications using MAB3010

• Elisa Pesce, Chiara Cordiglieri, Mauro Bombaci, Serenella Eppenberger-Castori, Stefania Oliveto, Cristina Manara et al. TMEM123 a key player in immune surveillance of colorectal cancer Frontiers in Immunology 2023-06-23 [PMID: 37426665]
• Elisa Pesce, Chiara Cordiglieri, Mauro Bombaci, Serenella Eppenberger-Castori, Stefania Oliveto, Cristina Manara et al. TMEM123 a key player in immune surveillance of colorectal cancer Frontiers in Immunology 2023-06-23 [PMID: 37426665] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: TMEM123
• Uniprot:
  • AAG21694()