Polycystin 2 Antibody (4C8) - Azide and BSA Free

Western Blot: Polycystin 2 Antibody (4C8) [H00005311-M01] - PKD2 monoclonal antibody (M01), clone 4C8. Analysis of PKD2 expression in human liver.

Immunocytochemistry/Immunofluorescence: Polycystin 2 Antibody (4C8) [H00005311-M01] - Analysis of monoclonal antibody to PKD2 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: Polycystin 2 Antibody (4C8) [H00005311-M01] - Detection limit for recombinant GST tagged PKD2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF
• Clone: 4C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Polycystin 2 Antibody (4C8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PKD2 (NP_000288, 261 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PVSKTEKTNFKTLSSMEDFWKFTEGSLLDGLYWKMQPSNQTEADNRSFIFYENLLLGVPRIRQLRVRNGSCSIPQDLRDEIKECYDVYSVSSEDRAPFGP
• Specificity: PKD2 - polycystic kidney disease 2 (autosomal dominant)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PKD2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. Use in PLA reported in secitific publication PMID: 32543276

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Polycystin 2 Antibody (4C8) - Azide and BSA Free

• APKD2
• Autosomal dominant polycystic kidney disease type II protein
• MGC138466
• MGC138468
• PC2
• Pc-2
• PKD4
• polycystic kidney disease 2 (autosomal dominant)
• Polycystic kidney disease 2 protein
• polycystin-2
• polycystwin
• R48321
• transient receptor potential cation channel, subfamily P, member 2
• TRPP2

Background

This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. The encoded protein may function in renal tubular development, morphology, and function, and may modulate intracellular calcium homoeostasis and other signal transduction pathways. This protein interacts with polycystin 1 to produce cation-permeable currents. Mutations in this gene have been associated with autosomal dominant polycystic kidney disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Polycystin 2 Antibody (H00005311-M01)(2)

Publications using H00005311-M01

• Keiichi T, Koji U, Masahiro S et al. Urinary extracellular vesicles signature for diagnosis of kidney disease. iScience. 2022-11-08 [PMID: 36439984]
• PeNa-Oyarzun D, Rodriguez-PeNa M, Burgos-Bravo F et al. PKD2/polycystin-2 induces autophagy by forming a complex with BECN1 Autophagy 2020-06-30 [PMID: 32543276] (PLA, Human)

Bioinformatics

• Gene Symbol: PKD2
• Entrez:
  • 5311(Human)
• OMIM:
  • 173910(Human)
• Uniprot:
  • NP_000288(Human)