Recombinant Human POLR2J His Protein

SDS-Page: Recombinant Human POLR2J His Protein [NBP2-23347] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human POLR2J His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids1-117 of Human POLR2J
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: POLR2J
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 15.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 40% glycerol, 5mM DTT, 2 mM EDTA
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human POLR2J His Protein

• DNA-directed RNA polymerase II subunit J-1
• DNA-directed RNA polymerase II subunit RPB11-a
• hRPB14
• MGC71910
• POLR2J1RNA polymerase II subunit B11-a
• polymerase (RNA) II (DNA directed) polypeptide J (13.3kD)
• polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
• RNA polymerase II 13.3 kDa subunit
• RPB11
• RPB11a
• RPB11m

Background

POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: POLR2J