PMCA1 Antibody

Immunohistochemistry-Paraffin: PMCA1 Antibody [NB120-3528] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunohistochemistry-Paraffin: PMCA1 Antibody [NB120-3528] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.

Product Discontinued

PMCA1 Antibody Summary

• Immunogen: Synthetic peptide corresponding to the residues A(5) N N S V A Y S G V K N S I K E A N(22) of rat PMCA1 ATPase.
• Localization: Cell Membrane; Multi-pass membrane protein
• Specificity: Detects PMCA 1a and 1b ATPase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP2B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 134 kDa and 130 kDa proteins representing PMCA 1a and 1b ATPase from rat samples.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PMCA1 Antibody

• ATPase, Ca++ transporting, plasma membrane 1
• EC 3.6.3
• EC 3.6.3.8
• Plasma membrane calcium ATPase isoform 1
• plasma membrane calcium ATPase
• Plasma membrane calcium pump isoform 1
• plasma membrane calcium pump
• plasma membrane calcium-ATPase
• PMCA1kb
• PMCA1plasma membrane calcium-transporting ATPase 1

Background

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PMCA1 Antibody (NB120-3528). (Showing 1 - 1 of 1 FAQ).

1. Does NB120-3528 cross-react with mouse? I am seeking one which has no cross-reactivity with mouse, but that has cross-reactivity with rat. I worry about the homology with rat and mouse. So, I need your advice. Could you let me know about it?
   • Regarding your concern with possible cross reactivity of NB120-3528 with mouse atp2b1, I did a blast search between the immunogen region of this antibody and mouse atp2b1 and it seems the homology is high (94%) suggesting there is cross reactivity, however it is very important to keep in mind that mouse atp2b1 is not a very well recognized protein and the sequencing information in UniProt is unreviewed meaning it has not been manually annotated and thus is not very reliable.

Bioinformatics

• Gene Symbol: ATP2B1
• Entrez:
  • 490(Human)
  • 29598(Rat)