Plexin B1 Antibody (559819) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: IHC
• Clone: 559819
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Plexin B1 Antibody (559819) [Alexa Fluor® 532] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Plexin B1
  Accession # O43157
• Specificity: Detects human Plexin B1 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin B1 Antibody (559819) [Alexa Fluor® 532]

• Plexin B1
• PLXN5
• PLXNB1
• SEP
• Transmembrane Protein SEP

Background

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors.  Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region.  The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats.  The ECD is cleaved into two subunits, a 200 kDa a-chain (aa 20-1305) and a 100 kDa TM b-chain.  The subunits are nondisulfide-linked and generate a high-affinity receptor.  Plexin B1 is a receptor for Semaphorin 4D/CD100.  It forms a receptor complex with Neuropilins, MET, and EGF R2.  Multiple splice variants are known. One shows a deletion of aa 689-871.  A second shows a 53 aa substitution for aa 677-2135.  And a third shows a 53 aa substitution for the 53 aa between aa 677-729.  Over aa 35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively.  

  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.