Plexin B1 Antibody (439512) [Unconjugated]

Plexin B1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Plexin B1 Monoclonal Antibody (Catalog # MAB3749) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 439512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Plexin B1 Antibody (439512) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Plexin B1
  Leu35-Val150
  Accession # O43157
• Specificity: Detects human Plexin B1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Plexin C1, rhPlexin D1, recombinant mouse (rm) Plexin A1, rmPlexin A2, or rmPlexin A3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLXNB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin B1 Antibody (439512) [Unconjugated]

• Plexin B1
• PLXN5
• PLXNB1
• SEP
• Transmembrane Protein SEP

Background

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha -chain (aa 20-1305) and a 100 kDa TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with Neuropilins, MET, and EGF R2. Multiple splice variants are known. One shows a deletion of aa 689-871. A second shows a 53 aa substitution for aa 677‑2135. And a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plexin B1 Antibody (MAB3749)(3)

Publications using MAB3749

• N Vilallongu, J Schaeffer, AM Hesse, C Delpech, B Blot, A Paccard, E Plissonnie, B Excoffier, Y Couté, S Belin, H Nawabi Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system Nature Communications, 2022;13(1):6040. 2022 [PMID: 36229455] (IHC, Mouse)
• Sun , Tianlian, Krishnan , Rameshku, Swiercz , Jakub M Grb2 mediates semaphorin-4D-dependent RhoA inactivation. J Cell Sci, 2012;125(0):3557-67. 2012 [PMID: 22505611] (Western Blot, Human)
• Worzfeld T, Swiercz JM, Looso M ErbB-2 signals through Plexin-B1 to promote breast cancer metastasis. J. Clin. Invest., 2012;122(4):1296-305. 2012 [PMID: 22378040] (Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: PLXNB1
• Uniprot:
  • O43157()