Plexin A1 Antibody (JU50-37)

Western Blot: Plexin A1 Antibody (JU50-37) [NBP2-75623] - Analysis of Plexin A1 on rat brain tissue lysates with Rabbit anti-Plexin A1 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 211 kDa Observed band size: 211 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (JU50-37) [NBP2-75623] - Staining Plexin A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: Plexin A1 Antibody (JU50-37) [NBP2-75623] - Analysis of LOVO cells with Plexin A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Discontinued

Plexin A1 Antibody (JU50-37) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Plexin A1 aa 100-200 / 1,896. (SwissProt: Q9UIW2 Human; SwissProt: P70206 Mouse)
• Localization: Cell Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLXNA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 211 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Plexin A1 Antibody (JU50-37)

• NOV
• NOVP
• Plexin A1
• PLEXIN-A1
• PLXN1
• PLXNA1

Background

Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLXNA1