Pleiotrophin/PTN Antibody (851406R) [Unconjugated]

Pleiotrophin/PTN was detected in immersion fixed PC-3 human prostate cancer cell line using Recombinant Rat Anti-Human Pleiotrophin/PTN Monoclonal Antibody (Catalog # MAB252R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ELISA, ICC/IF
• Clone: 851406R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Pleiotrophin/PTN Antibody (851406R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN
  Gly33-Asp168
  Accession # P21246
• Specificity: Detects human Pleiotrophin in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: PTN
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pleiotrophin/PTN Antibody (851406R) [Unconjugated]

• HARP
• HBBM
• HB-GAM
• HBGF8
• HBNF
• HBNF1
• HBNF-1
• Heparin-binding brain mitogen
• HNGF-8
• NEGF1HBGF-8
• neurite growth-promoting factor 1
• neurite growth-promoting factor1)
• OSF-1
• Osteoblast-specific factor 1
• Pleiotrophin
• PTN

Background

Pleiotrophin (PTN), also called heparin-binding growth-associated molecule (HB-GAM), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), or osteoblast-specific factor (OSF-1), is an 18 kDa secreted, strongly heparin-binding, developmentally regulated cytokine (1-3). PTN and Midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family (1-3). The second of two TSP1 domains contains the highest affinity binding site for heparin (4, 5). A 15 kDa form which lacks the C-terminus is mitogenic for glioblastoma cells, while full-length PTN is not (6). PTN is a highly conserved protein; human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity or greater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis (3). In the adult, it is induced by PDGF and upregulated in many cancers, hematopoietic stem cells and tissues undergoing remodeling (7-10). Cell surface receptors for PTN include Syndecan-3 (which mediates neurite outgrowth) and the receptor tyrosine phosphatase PTPRB, also called RPTP beta / zeta (3, 11-13). Heparin binding is necessary for engaging these receptors (7, 8). PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates (12-14). One such substrate is the WNT pathway molecule beta -catenin, allowing crosstalk of PTN with WNTs (12). PTN activation of the receptor ALK (anaplastic lymphoma kinase) is indirect through PTPRB, and mediates mitogenic, transforming and angiogenic activities of PTN (2, 5, 6, 13). Increased expression of PTN is correlated with neuronal development or stresses such as brain ischemia and Parkinson’s disease (2, 3, 7, 8). Both PTN and Midkine have demonstrated bactericidal activity, but only in the absence of heparin (15).

1. Naito, A. et al. (1992) Biochem. Biophys. Res. Commun. 183:701.
2. Perez-Pinera, P. et al. (2008) Curr. Opin. Hematol. 15:210.
3. Weng, T. and L. Liu (2010) Respir. Res. 11:80.
4. Raulo, E. et al. (2005) J. Biol. Chem. 280:41576.
5. Hamma-Kourbali, Y. et al. (2008) J. Cell. Physiol. 214:250.
6. Lu, K.V. et al. (2005) J. Biol. Chem. 280:16953.
7. Yeh, H-J. et al. (1998) J. Neurosci. 18:3699.
8. Marchionini, D.M. et al. (2007) Brain Res. 1147:77.
9. Chang, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10888.
10. Himburg, H.A. et al. (2010) Nat. Med. 16:475.
11. Kinnunen, T. et al. (1996) J. Biol. Chem. 271:2243.
12. Meng, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2603.
13. Perez-Pinera, P. et al. (2007) J. Biol. Chem. 282:28683.
14. Fukada, M. et al. (2006) FEBS Lett. 580:4051.
15. Svensson, S.L. et al. (2010) J. Biol. Chem. 285:16105.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTN
• Uniprot:
  • P21246()