Pleiotrophin/PTN Antibody (851303R) [Unconjugated]


There are currently no images for Pleiotrophin/PTN Antibody (MAB2522).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications ELISA

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Pleiotrophin/PTN Antibody (851303R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Pleiotrophin/PTN
Accession # P21246
Detects human Pleiotrophin/PTN in direct ELISAs.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.



Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pleiotrophin/PTN Antibody (851303R) [Unconjugated]

  • HARP
  • HBBM
  • HB-GAM
  • HBGF8
  • HBNF
  • HBNF1
  • HBNF-1
  • Heparin-binding brain mitogen
  • HNGF-8
  • neurite growth-promoting factor 1
  • neurite growth-promoting factor1)
  • OSF-1
  • Osteoblast-specific factor 1
  • Pleiotrophin
  • PTN


Pleiotrophin (PTN), also called heparin-binding growth-associated molecule (HB-GAM), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), or osteoblast-specific factor (OSF-1), is an 18 kDa secreted, strongly heparin-binding, developmentally regulated cytokine (1 ‑ 3). PTN and Midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family (1 ‑ 3). The second of two TSP1 domains contains the highest affinity binding site for heparin (4, 5). A 15 kDa form which lacks the C-terminus is mitogenic for glioblastoma cells, while full-length PTN is not (6). PTN is a highly conserved protein; human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity or greater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis (3). In the adult, it is induced by PDGF and upregulated in many cancers, hematopoietic stem cells and tissues undergoing remodeling (7 ‑ 10). Cell surface receptors for PTN include Syndecan-3 (which mediates neurite outgrowth) and the receptor tyrosine phosphatase PTPRB, also called RPTP beta / zeta (3, 11 ‑ 13). Heparin binding is necessary for engaging these receptors (7, 8). PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates (12 ‑ 14). One such substrate is the WNT pathway molecule beta -catenin, allowing crosstalk of PTN with WNTs (12). PTN activation of the receptor ALK (anaplastic lymphoma kinase) is indirect through PTPRB, and mediates mitogenic, transforming and angiogenic activities of PTN (2, 5, 6, 13). Increased expression of PTN is correlated with neuronal development or stresses such as brain ischemia and Parkinson’s disease (2, 3, 7, 8). Both PTN and Midkine have demonstrated bactericidal activity, but only in the absence of heparin (15).
  1. Naito, A. et al. (1992) Biochem. Biophys. Res. Commun. 183:701.
  2. Perez-Pinera, P. et al. (2008) Curr. Opin. Hematol. 15:210.
  3. Weng, T. and L. Liu (2010) Respir. Res. 11:80.
  4. Raulo, E. et al. (2005) J. Biol. Chem. 280:41576.
  5. Hamma-Kourbali, Y. et al. (2008) J. Cell. Physiol. 214:250.
  6. Lu, K.V. et al. (2005) J. Biol. Chem. 280:16953.
  7. Yeh, H-J. et al. (1998) J. Neurosci. 18:3699.
  8. Marchionini, D.M. et al. (2007) Brain Res. 1147:77.
  9. Chang, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10888.
  10. Himburg, H.A. et al. (2010) Nat. Med. 16:475.
  11. Kinnunen, T. et al. (1996) J. Biol. Chem. 271:2243.
  12. Meng, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2603.
  13. Perez-Pinera, P. et al. (2007) J. Biol. Chem. 282:28683.
  14. Fukada, M. et al. (2006) FEBS Lett. 580:4051.
  15. Svensson, S.L. et al. (2010) J. Biol. Chem. 285:16105.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu
Applications: WB, Simple Western, IP, ICC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP
Species: Hu
Applications: WB
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
Species: Hu
Applications: WB
Species: Mu
Applications: WB, IHC
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ELISA, IHC, IHC-P
Species: Hu, Mu, Rt, Ca, Eq

Publications for Pleiotrophin/PTN Antibody (MAB2522) (0)

There are no publications for Pleiotrophin/PTN Antibody (MAB2522).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Pleiotrophin/PTN Antibody (MAB2522) (0)

There are no reviews for Pleiotrophin/PTN Antibody (MAB2522). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Pleiotrophin/PTN Antibody (MAB2522) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Array Products

Diseases for Pleiotrophin/PTN Antibody (MAB2522)

Discover more about diseases related to Pleiotrophin/PTN Antibody (MAB2522).

Pathways for Pleiotrophin/PTN Antibody (MAB2522)

View related products by pathway.

PTMs for Pleiotrophin/PTN Antibody (MAB2522)

Learn more about PTMs related to Pleiotrophin/PTN Antibody (MAB2522).

Blogs on Pleiotrophin/PTN

There are no specific blogs for Pleiotrophin/PTN, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Pleiotrophin/PTN Antibody (851303R) [Unconjugated] and receive a gift card or discount.