PLCD4 Antibody (4D4) - Azide and BSA Free

Western Blot: PLCD4 Antibody (4D4) [H00084812-M01] - Analysis of PLCD4 expression in transfected 293T cell line by PLCD4 monoclonal antibody (M01), clone 4D4.Lane 1: PLCD4 transfected lysate (Predicted MW: 87.6 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: PLCD4 Antibody (4D4) [H00084812-M01] - Detection limit for recombinant GST tagged PLCD4 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PLCD4 Antibody (4D4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PLCD4 Antibody (4D4) - Azide and BSA Free (H00084812-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PLCD4 (AAH06355, 18 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVT
• Localization: Membrane; Peripheral membrane protein. Nucleus. Cytoplasm. Endoplasmic reticulum. Note=Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.
• Specificity: PLCD4 - phospholipase C, delta 4
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLCD4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLCD4 Antibody (4D4) - Azide and BSA Free

• EC 3.1.4.11
• hPLCD4
• MGC12837
• Phosphoinositide phospholipase C-delta-4
• phospholipase C, delta 4
• Phospholipase C-delta-4
• PLC delta4
• PLC-delta-4,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-4

Background

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression upregulates the Erk signaling pathway and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCD4
• Entrez:
  • 84812(Human)
• OMIM:
  • 605939(Human)
• Uniprot:
  • AAH06355(Human)