PLCD4 Antibody (4D4)

Images

 
Western Blot: PLCD4 Antibody (4D4) [H00084812-M01] - Analysis of PLCD4 expression in transfected 293T cell line by PLCD4 monoclonal antibody (M01), clone 4D4.Lane 1: PLCD4 transfected lysate (Predicted MW: 87.6 ...read more
Sandwich ELISA: PLCD4 Antibody (4D4) [H00084812-M01] - Detection limit for recombinant GST tagged PLCD4 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PLCD4 Antibody (4D4) Summary

Immunogen
PLCD4 (AAH06355, 18 a.a. ~ 128 a.a) partial recombinant protein with GST tag.MQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKP SFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRSN LDLMANSVEEAQIWMRGLQLLVDLVT
Localization
Membrane; Peripheral membrane protein. Nucleus. Cytoplasm. Endoplasmic reticulum. Note=Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.
Specificity
PLCD4 - phospholipase C, delta 4
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PLCD4
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLCD4 Antibody (4D4)

  • EC 3.1.4.11
  • hPLCD4
  • MGC12837
  • Phosphoinositide phospholipase C-delta-4
  • phospholipase C, delta 4
  • Phospholipase C-delta-4
  • PLC delta4
  • PLC-delta-4,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-4

Background

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression upregulates the Erk signaling pathway and proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PLCD4
Entrez
OMIM
Uniprot