PLA2R1 Antibody

Flow Cytometry: PLA2R1 Antibody [NBP1-46116] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Po, Ca
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PLA2R1 Antibody Summary

• Description: Novus Biologicals Goat PLA2R1 Antibody (NBP1-46116) is a polyclonal antibody validated for use in WB, ELISA and Flow. Anti-PLA2R1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKKKDTPKQHGT corresponding to internal region according to NP_031392.3, NP_001007268.1, NP_001182570.1.
• Specificity: This antibody is expected to recognize all reported isoforms (NP_031392.3; NP_001007268.1; NP_001182570.1).
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLA2R1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Peptide ELISA 1:1000
  • Western Blot
• Application Notes: Use in Western blot reported in scientific literature (PMID: 24500714).
• Theoretical MW: 153 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PLA2R1 Antibody

• 180 kDa secretory phospholipase A2 receptor
• CLEC13C
• CLEC13CC-type lectin domain family 13 member C
• phospholipase A2 receptor 1, 180kDa
• PLA2G1R
• PLA2IR
• PLA2R1
• PLA2-RM-type receptor
• PLA2Rphospholipase A2 receptor 1, 180kD
• secretory phospholipase A2 receptor

Background

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLA2R1 Antibody (NBP1-46116)(2)

Publications using NBP1-46116

• Jurgensen HJ, Johansson KB, Madsen DH et al. Complex Determinants in Specific Members of the Mannose Receptor Family Govern Collagen Endocytosis. J. Biol. Chem. 2014-02-06 [PMID: 24500714] (WB, Human)
• Silliman CC, Moore EE, Zallen G et al. Presence of the M-type sPLA(2) receptor on neutrophils and its role in elastase release and adhesion. Am J Physiol Cell Physiol 2002-10-01 [PMID: 12225974]

Bioinformatics

• Gene Symbol: PLA2R1
• Entrez:
  • 22925(Human)
• Uniprot:
  • Q13018(Human)