PLA2G7/PAF-AH/Lp-PLA2 Antibody

Product Discontinued

PLA2G7/PAF-AH/Lp-PLA2 Antibody Summary

• Immunogen: Peptide with sequence C-QTKIPRGNGPYS corresponding to internal region according to NP_005075.3.
• Specificity: Reported variants represent identical protein: NP_005075.3, NP_001161829.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PLA2G7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 100 kDa band in human liver lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.1 kDa band according to NP_005075.3. The 100 kDa band was successfully blocked by incubation with the immunizing peptide. Does the observed molecular weight correspond to a glycosylated form?

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.

Alternate Names for PLA2G7/PAF-AH/Lp-PLA2 Antibody

• 2-acetyl-1-alkylglycerophosphocholine esterase
• EC 3.1.1
• EC 3.1.1.47,1-alkyl-2-acetylglycerophosphocholine esterase
• Group-VIIA phospholipase A2
• gVIIA-PLA2
• LDL-associated phospholipase A2
• LDL-PLA(2)
• LDL-PLA2
• lipoprotein-associated phospholipase A2
• LpPLA2
• Lp-PLA2
• PAF acetylhydrolase
• PAF-AH
• PAFAHPAF 2-acylhydrolase
• phospholipase A2, group VII (platelet-activating factor acetylhydrolase
• PLA2G7
• plasma)
• platelet-activating factor acetylhydrolase

Background

The protein encoded by the PLA2G7 gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLA2G7/PAF-AH/Lp-PLA2 Antibody (NBP2-26097)(1)

Publication using NBP2-26097

• Moldoveanu E, Serban M, Marta DS et al. Lipoprotein-associated phospholipase A2 activity in patients with preserved left ventricular ejection fraction. Biomarkers 2011-11-01 [PMID: 21942556]

Bioinformatics

• Gene Symbol: PLA2G7
• Entrez:
  • 7941(Human)