PLA2G2A Assay Kit (Colorimetric)

• Reactivity: Mu
• Applications: ELISA, Func
• Suitable Sample Type: Tissue Homogenates, Cell Lysates
• Standard Curve Range: 3.5 to 42 nmol/min/ml

PLA2G2A Assay Kit (Colorimetric) Summary

• Description: Phospholipases A2 (calcium-dependent cytosolic) Assay Kit is a colorimetric method for assaying the activity of cPLA2.
• Specificity: Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone unless specific inhibitors or purification procedures are used.
• Standard Curve Range: 3.5 to 42 nmol/min/ml
• Assay Type: Colorimetric
• Sample Volume: 10 uL/well. All samples should be assayed in duplicate at minimum.
• Kit Type: Assay Kit (Colorimetric)
• Gene: PLA2G2A

Applications/Dilutions

• Dilutions:
  • ELISA
  • Functional

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLA2G2A Assay Kit (Colorimetric)

• EC 3.1.1.4
• GIIC sPLA2
• Group IIA phospholipase A2
• Mom1
• Non-pancreatic secretory phospholipase A2
• NPS-PLA2
• Phosphatidylcholine 2-acylhydrolase 2A
• phospholipase A2, group IIA (platelets, synovial fluid)
• phospholipase A2, membrane associated
• PLA2B
• PLA2BPLA2
• PLA2G2A
• PLA2L
• PLA2LPLA2S
• PLAS1
• RASF-A
• sPLA2

Background

Phospholipases A2 (PLA2s) catalyze the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids, yielding a free fatty acid and a lysophospholipid as products. The release of arachidonic acid from membrane phospholipids by these enzymes is believed to be the key step in the biosynthesis of eicosanoids. There are primarily three different kinds of PLA2s, they are secretory (sPLA2), calcium-dependent cytosolic (cPLA2), and calcium-independent (iPLA2) PLA2s. Of these three different types of enzymes, only the cPLA2 exhibits specificity towards arachidonic acid whereas all others can hydrolyze any fatty acid at the sn-2 position.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for PLA2G2A Kit (KA1307)(1)

Publication using KA1307

• Wang X, Shaw DK, Hammond HL et al. The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation. PLoS Pathog 2016-08-02 [PMID: 27482714]

Bioinformatics

• Gene Symbol: PLA2G2A