PLA2G2A Antibody (620501) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IP
• Clone: 620501
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

PLA2G2A Antibody (620501) [Alexa Fluor® 700] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PLA2G2A
  Asn21-Cys144
  Accession # P14555
• Specificity: Detects human PLA2G2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human PLA2G1B, G2A, or G7 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLA2G2A Antibody (620501) [Alexa Fluor® 700]

• EC 3.1.1.4
• GIIC sPLA2
• Group IIA phospholipase A2
• Mom1
• Non-pancreatic secretory phospholipase A2
• NPS-PLA2
• Phosphatidylcholine 2-acylhydrolase 2A
• phospholipase A2, group IIA (platelets, synovial fluid)
• phospholipase A2, membrane associated
• PLA2B
• PLA2BPLA2
• PLA2G2A
• PLA2L
• PLA2LPLA2S
• PLAS1
• RASF-A
• sPLA2

Background

Phospholipase A2 Group IIA (PLA2G2A) is a 17 kDa member of the phospholipase A2 family of enzymes that hydrolyzes the sn-2 ester bond of phospholipids. Human PLA2G2A is synthesized as a 144 amino acid (aa) precursor that contains a 20 aa signal sequence and a 124 aa mature chain. Mature human PLA2G2A shares 72%, 68%, and 60% aa sequence identity with mature rat, mouse, and bovine PLA2G2A, respectively. PLA2G2A is a calcium dependent phospholipase expressed in many cell types associated with inflammation, including platelets, neutrophils, and mast cells. Most secretory PLA2s are stored in cytoplasmic granules and are released into the extracellular environment on appropriate cell activation. Thus, they are present at higher concentrations in the plasma and biologic fluids of patients with systemic inflammatory, autoimmune, or allergic diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.