PLA2G16/HRASLS3 Antibody [Alexa Fluor® 488]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

PLA2G16/HRASLS3 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PLA2G16
  Asp12-Asp132
  Accession # P53816
• Specificity: Detects human PLA2G16 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PLA2G16 (Group 16 phospholipase A2; also HREV107 and AdPLA) is a Ca⁺⁺‑dependent 17‑18 kDa member of the H‑rev107 family of molecules. It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids. The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels. This results in decreased hormone‑sensitive lipase activity and increased adiposity. Human PLA216 is 162 amino acids (aa) in length. It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa 134‑154. The enzymatic activity resides in His23 and Cys113. There is one potential isoform that shows a 20 aa substitution for the first four amino acids. Over aa 12 ‑ 132, human PLA2G16 shares 88% aa identity with mouse PLA2G16.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.