PIWIL2 Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

PIWIL2 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human PIWIL2
  Lys216-Asp379
  Accession # Q8TC59
• Specificity: Detects human PIWIL2 in direct ELISAs and human and rat PIWIL2 in Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human PIWIL4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIWIL2 Antibody [Alexa Fluor® 405]

• Cancer/testis antigen 80
• CT80
• CT80mili
• FLJ10351
• HILI
• HILIMiwi like
• MGC133049
• MILI
• PIWIL1L
• PIWIL2
• piwil2-like protein
• piwi-like 2 (Drosophila)
• piwi-like protein 2

Background

PIWIL2 (PIWI [P-element-induced wimpy testis]-like 2; also HILI) is a 100-110 kDa cytosolic member of the PIWI subfamily, Argonaute family of proteins. It is expressed in the germ line, particularly in premeiotic and early meiotic germ cells, plus mesenchymal stem cells, and in select cancer stem cells such as CD44⁺CD24^- breast cancer cells. PIWIL2 likely silences mobile genetic elements in germ cells, and appears to depress stem cell proliferation by inhibiting the expression of cell cycle genes. Human PIWIL2 is 973 amino acids (aa) in length. It contains one PAZ domain (aa 384-496) and a C-terminal PIWI domain (aa 668-959) that interact with RNA. There are multiple methylation sites on arginines. Two potential splice forms are reported, one that shows a deletion of aa 887-922, and a second that contains a 12 aa substitution for aa 486-801. Over aa 216-379, human PIWIL2 shares 96% aa identity with rat PIWIL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.