PIP5K1 alpha Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-44148] - Staining of human Tonsil Tissue.

Immunohistochemistry of PIP5K1 alpha Antibody. Tissue: Tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PIP5K1A antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIP5K1A as precipitated red signal with hematoxylin purple nuclear counterstain.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PIP5K1 alpha Antibody - Azide and BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: PIP5K1 alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PIP5K1 alpha protein. (Uniprot: Q99755)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIP5K1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1ug/ml
• Application Notes: This product is tested by IHC and ELISA useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62.6kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with PI4K, type I alpha from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for PIP5K1 alpha Antibody - Azide and BSA Free

• 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha
• EC 2.7.1
• EC 2.7.1.68
• Phosphatidylinositol-4-phosphate 5-kinase type I alpha
• phosphatidylinositol-4-phosphate 5-kinase type-1 alpha
• phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
• PIP5K1-alpha
• PIP5KIalpha
• PtdIns(4)P-5-kinase 1 alpha

Background

Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIP5K1A
• Uniprot:
  • Q99755(Human)