PIKFyve/PIP5K3 Antibody

Western Blot: PIKFyve/PIP5K3 Antibody [NBP3-12608] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:237 kDaObserved band size:237 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody [NBP3-12608] - Staining of human PIKFYVE in human kidney with rabbit polyclonal antibody at 1:300 dilution.

Immunoprecipitation: PIKFyve/PIP5K3 Antibody [NBP3-12608] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-PIKFYVE rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PIKFYVE rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 237 kDaObserved band size :237 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PIKFyve/PIP5K3 Antibody Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PIKFyve/PIP5K3 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIKFyVE
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PIKFyve/PIP5K3 Antibody

• 1-phosphatidylinositol-3-phosphate 5-kinase
• EC 2.7.1.150
• FAB1
• FLJ37746
• FYVE finger-containing phosphoinositide kinase
• KIAA0981CFD
• MGC40423
• p235
• Phosphatidylinositol-3-phosphate 5-kinase type III
• Phosphatidylinositol-3-phosphate 5-kinase
• phosphoinositide kinase, FYVE finger containing
• PIKFyve
• PIP5K
• PIP5K3
• PIP5K3FAB1
• PIPkin-III
• Type III PIP kinase
• type III

Background

PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. Signaling by phosphorylated species of phosphatidylinositol regulates diverse cellular processes, including membrane trafficking and cytoskeletal reorganization (Shisheva et al., 1999 [PubMed 9858586]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIKFyVE