PIKFyve/PIP5K3 Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human PIKFyve Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7885) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIKFyve at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PIKFyve/PIP5K3 Antibody Summary

• Immunogen: E. coli-derived recombinant human PIKFyve
  Lys215-Lys361
  Accession # Q9Y2I7
• Specificity: Detects human PIKFyve in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PIKFYVE
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIKFyve/PIP5K3 Antibody

• 1-phosphatidylinositol-3-phosphate 5-kinase
• EC 2.7.1.150
• FAB1
• FLJ37746
• FYVE finger-containing phosphoinositide kinase
• KIAA0981CFD
• MGC40423
• p235
• Phosphatidylinositol-3-phosphate 5-kinase type III
• Phosphatidylinositol-3-phosphate 5-kinase
• phosphoinositide kinase, FYVE finger containing
• PIKFyve
• PIP5K
• PIP5K3
• PIP5K3FAB1
• PIPkin-III
• Type III PIP kinase
• type III

Background

PIKfyve (PhosphoInositide Kinase for five position containing a fyve finger (FAB1/YOTB/Vac1/EEA1) domain; also phosphatidylinositol 3-phosphate 5-kinase type III and Type III PIP kinase) is a 230-260 kDa cytoplasmic and endocytic member of an ancient family of phosphatidylinositol-3,5 biphosphate (PI[3,5]P2 synthesizing enzymes. It is associated with endosomes, particularly the microdomains of early endosomes that are EEA1-deficient. PIKfyve binds to membrane-embedded phosphatidylinositol and initially converts PI3P into PI(3,5)P2, which is then converted into PI5P. In conjunction with ArPIKfyve and Sac3, its actions are involved in the normal transport, fusion, and membrane export of endosomes. Notably, PIKfyve also appears to act as a protein kinase, apparently controlling its own activity through autophosphorylation. Human PIKfyve is 2098 amino acids (aa) in length. It contains one FYVE PI3P-binding zinc-finger domain (aa 154-219), a DEP domain (aa 365-440) and a C-terminal PIP kinase rehion (aa 1172-2085). There are no less than 29 utilized Ser/Thr phosphorylation sites, plus two utilized Tyr phosphorylation sites. Three potential isoform variants are reported, all which possess a three aa substitution for aa 546-2098. Two of these three also demonstrate a deletion of aa 108-204, and a nine aa substitution for aa 109-203, respectively. Over aa 215-361, human and mouse share 95% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIKFyve/PIP5K3 Antibody (AF7885)(3)

Publications using AF7885

• Koike, S;Jahn, R; Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic Nature communications 2024-03-20 [PMID: 38509070] (Western Blot, Human)
• Yuanyuan Qiao, Jae Eun Choi, Jean C. Tien, Stephanie A. Simko, Thekkelnaycke Rajendiran, Josh N. Vo et al. Autophagy inhibition by targeting PIKfyve potentiates response to immune checkpoint blockade in prostate cancer Nature Cancer 11/5/2021 [PMID: 34738088]
• V Sharma, M Makhdoomi, L Singh, P Kumar, N Khan, S Singh, HN Verma, K Luthra, S Sarkar, D Kumar Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block Autophagy, 2020-02-20;0(0):1-20. 2020-02-20 [PMID: 32079455] (Human)

Bioinformatics

• Gene Symbol: PIKFYVE
• Uniprot:
  • Q9Y2I7(Human)