PIGY Antibody - BSA Free

Western Blot: PIGY Antibody [NBP1-77340] - Analysis of PIG-Y in human liver tissue lysate with PIG-Y antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/ Immunofluorescence: PIGY Antibody - BSA Free [NBP1-77340] - Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 u/mL.

Immunohistochemistry: PIGY Antibody - BSA Free [NBP1-77340] - Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PIGY Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PIGY Antibody - BSA Free (NBP1-77340) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y. The immunogen is located within amino acids 50 - 100 of PIG-Y. Amino Acid Squence: CPLSKKPLRYEASTNE
• Specificity: This antibody only detects isoform 1 of PIG-Y.
• Predicted Species: Rat (94%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIGY
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PIGY Peptide (NBP1-77340PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PIGY Antibody - BSA Free

• Phosphatidylinositol-glycan biosynthesis class Y protein
• PIG-Y

Background

Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIGY
• Uniprot:
  • NP_116295(Human)