PIGY Antibody

Images

 
Western Blot: PIGY Antibody [NBP1-76967] - Analysis of PIG-Y in human skeletal muscle tissue lysate with PIG-Y antibody at 5 ug/mL.
Immunocytochemistry/ Immunofluorescence: PIGY Antibody [NBP1-76967] - Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: PIGY Antibody [NBP1-76967] - Human spleen tissue with PIGY antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PIGY Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PIG-Y. The immunogen is located within the last 50 amino acids of PIG-Y.
Specificity
This antibody only detects isoform 1 of PIG-Y.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIGY
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
PIG-Y antibody can be used for detection of PIG-Y by Western blot at 5 ug/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL.
Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PIGY Peptide (NBP1-76967PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIGY Antibody

  • Phosphatidylinositol-glycan biosynthesis class Y protein
  • PIG-Y

Background

Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIGY
Uniprot